Description
Phospho-LAT (Tyr220) (E3S5L) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of LAT protein when phosphorylated at Tyr220. This antibody shows cross-reactivity with phosphorylated EGFR, and detects a 70 kDa band of unknown origin in some treated cell lines. Tyr220 of LAT, long isoform corresponds to Tyr191 of LAT, short isoform. Weak cross-reactivity with phosphorylated tyrosines 171 and 255 of LAT has been observed. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr220 of human LAT protein.
Target
LAT (Tyr220) phosphate
Target Alias Name
LAT
Isotype/Mimetic
IgG
Animal-Derived Biomaterials Used
None
Sequence Available
Yes
Original Discovery Method
Immunization
Antibody/Binder Origins
Animal-dependent discovery, post-2020, In vitro recombinant expression