Description
Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of histone H3 only when phosphorylated at Ser10. The antibody does not cross-react with other phosphorylated histones or with acetylated histones.
Lack of staining in G2 cells has been reported in some cell lines by immunofluorescence. Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3. This antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.
Target
H3 (Ser10) phosphate
Target Alias Names
H3C1, H3
Isotype/Mimetic
IgG
Animal-Derived Biomaterials Used
BSA
Sequence Available
Yes
Original Discovery Method
Immunization
Antibody/Binder Origins
Animal-dependent discovery (in vitro display, OR immunisation pre-2020), In vitro recombinant expression, Animal-derived biomaterials used in production or final formulation