Description
Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb (BSA and Azide Free) detects endogenous levels of histone H3 only when mono-methylated on Lys4. The antibody does not cross-react with non-methylated, di-methylated or tri-methylated histone H3 Lys4. In addition, the antibody does not cross-react with methylated histone H3 Lys9, Lys27, Lys36 or methylated histone H4 Lys20. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which lysine 4 is mono-methylated.
Target
H3 (Lys4) methylate
Target Alias Names
H3C1, H3
Isotype/Mimetic
IgG
Animal-Derived Biomaterials Used
None
Sequence Available
Yes
Original Discovery Method
Immunization
Antibody/Binder Origins
Animal-dependent discovery (in vitro display, OR immunisation pre-2020), In vitro recombinant expression