Description
This product is a recombinant humanized version of the OGD1.0.0 antibody that recognizes MSTN. The CDR2 amino acid sequence from the humanized VH0 and VL0 regions were compared to the CDR2 sequence from the human germline VH region DP-47 and VL region DPK-9, respectively. All residues in the VH0 and VL0 CDR2 sequences differing from the human sequence were changed to human. The new VH and VL regions were designated VH2 and VL2, respectively. Based on the sequence of CDR2 in VL2, position 50 was substituted with alanine (550A) to create VL4. OGD1.0.4 antibody was produced using VH0 and VL4 and retained substantial activity.
Target
MSTN
Target Alias Names
Myostatin, GDF8, Growth Differentiation Factor 8, Growth/Differentiation Factor 8, MSLHP, GDF-8
Isotype/Mimetic
Humanized IgG
Animal-Derived Biomaterials Used
None
Sequence Available
No
Original Discovery Method
Human-derived phage display
Original Discovery Information Provided On Datasheet
No
Antibody/Binder Origins
Animal-free discovery, In vitro recombinant expression