Description
anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) (ATTO 488) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain and labeled with ATTO 488. anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain). Species cross-reactivity: Drosophila, Human, Mouse, Rat. Clone: SF9. Isotype: Human IgG2lambda. Applications: ICC. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Target
Myosin IIA (heavy chain)
Target Alias Names
Cellular Myosin Heavy Chain, Type A, Myosin Heavy Chain 9, Myosin Heavy Chain, Non-muscle IIa, Non-muscle Myosin Heavy Chain A
Isotype/Mimetic
Human IgG2lambda
Animal-Derived Biomaterials Used
None
Sequence Available
Yes
Original Discovery Method
Human-derived phage display
Original Discovery Information Provided On Datasheet
Yes
Antibody/Binder Origins
Animal-free discovery, In vitro recombinant expression