Description
anti-Isthmin-1, mAb (rec.) (Giusepi-1-4) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the mouse lgG2b Fc domain. Anti-Isthmin-1 Monoclonal Antibody (recombinant) (Giusepi-1-4) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. Recognizes human and mouse Isthmin-1. Clone: Giusepi-1-4. Isotype: Mouse IgG2blambda. Applications: ELISA, WB. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS and 0.02% Proclin-300. Isthmin-1 is important for embryonic and postnatal development. Growing evidence has shown that aberrant expression of Isthmin-1 can also affect the biological behavior of cancer.
Target
Isthmin-1
Target Alias Name
C20orf82
Isotype/Mimetic
Mouse IgG2blambda
Animal-Derived Biomaterials Used
None
Sequence Available
Yes
Original Discovery Method
Human-derived phage display
Original Discovery Information Provided On Datasheet
Yes
Antibody/Binder Origins
Animal-free discovery, In vitro recombinant expression