Description
5-Formylcytosine (5-fC) (D5D4K) Rabbit mAb detects 5-fC by IF in cells over-expressing the TET1 catalytic domain and by dot blot using double-stranded PCR fragments containing 5-fC. Many cells and tissues contain very low endogenous levels of 5-fC that may fall below the detection limits of this antibody. This antibody has been validated for specificity using ELISA and dot blot and shows high specificity for 5-fC. Monoclonal antibody is produced by immunizing animals with 5-formyl-2'-deoxycytosine.
Target
5-formylcytosine
Isotype/Mimetic
IgG
Animal-Derived Biomaterials Used
BSA
Sequence Available
Yes
Original Discovery Method
Immunization
Antibody/Binder Origins
Animal-dependent discovery (in vitro display, OR immunisation pre-2020), In vitro recombinant expression, Animal-derived biomaterials used in production or final formulation