Description
5-Carboxylcytosine (5-caC) (D7S8U) Rabbit mAb detects 5-caC by IF in cells over-expressing the TET1 catalytic domain and by dot blot using double-stranded PCR fragments containing 5-caC. Many cells and tissues contain very low endogenous levels of 5-caC that may fall below the detection limits of this antibody. This antibody has been validated using ELISA, dot blot, and synthetic spike-in DNA MeDIP assays and shows high specificity for 5-caC. Monoclonal antibody is produced by immunizing animals with 5-carboxylcytidine.
Target
5-carboxylcytosine
Isotype/Mimetic
IgG
Animal-Derived Biomaterials Used
BSA
Sequence Available
Yes
Original Discovery Method
Immunization
Antibody/Binder Origins
Animal-dependent discovery (in vitro display, OR immunisation pre-2020), In vitro recombinant expression, Animal-derived biomaterials used in production or final formulation