Description
3X DYKDDDDK Tag (E7C5T) Rabbit mAb detects exogenously expressed proteins containing the 3X DYKDDDDK epitope tag. This antibody recognizes the 3X DYKDDDDK tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells, but does not detect DYKDDDDK tag. 3X DYKDDDDK Tag (E7C5T) Rabbit mAb detects exogenously expressed 3X DYKDDDDK-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the 3X DYKDDDDK epitope (DYKDHDGDYKDHDIDYKDDDDK).
Target
3x Flag-Tag
Isotype/Mimetic
IgG
Animal-Derived Biomaterials Used
BSA
Sequence Available
Yes
Original Discovery Method
Immunization
Antibody/Binder Origins
Animal-dependent discovery, post-2020, In vitro recombinant expression, Animal-derived biomaterials used in production or final formulation