Description
Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of caspase-3 protein only when cleaved at Asp175. This antibody is preferred for immunofluorescence, flow cytometry, and immunohistochemistry applications. This antibody detects non-specific caspase substrates by western blot. Non-specific labeling may be observed by immunofluorescence in specific sub-types of healthy cells in fixed-frozen tissues (e.g., pancreatic alpha-cells). Nuclear background may be observed in rat and monkey samples. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human caspase-3 protein.
Target
Casp3 (Asp175) cleaved
Target Alias Name
CASP3
Isotype/Mimetic
IgG
Animal-Derived Biomaterials Used
None
Sequence Available
Yes
Original Discovery Method
Immunization
Antibody/Binder Origins
Animal-dependent discovery (in vitro display, OR immunisation pre-2020), In vitro recombinant expression