Description
Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb (BSA and Azide Free) detects endogenous levels of histone H3 when di-methylated on Lys4. This antibody shows weak cross-reactivity with histone H3 that is mono-methylated on Lys4 but does not cross-react with non-methylated or tri-methylated histone H3 Lys4. In addition, the antibody does not cross-react with methylated histone H3 Lys9, Lys27, Lys36 or histone H4 Lys20. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys4 is di-methylated.
Target
H3 (Lys4) dimethylate
Target Alias Names
H3C1, H3
Isotype/Mimetic
IgG
Animal-Derived Biomaterials Used
None
Sequence Available
Yes
Original Discovery Method
Immunization
Antibody/Binder Origins
Animal-dependent discovery (in vitro display, OR immunisation pre-2020), In vitro recombinant expression