Description
Phospho-14-3-3 ζ/δ (Ser58)/η (Ser59)/γ (Ser59)/β/α (Ser60) (E6B3G) Rabbit mAb detects endogenous levels of 14-3-3 ζ/δ only when phosphorylated at Ser58, 14-3-3 η and 14-3-3 γ only when phosphorylated at Ser59, and 14-3-3 α/β only when phosphorylated at Ser60. This antibody does not cross-react with 14-3-3 ε, 14-3-3 θ, or 14-3-3 σ. This antibody also recognizes a non-specific band of unknown origin at 80 kDa and 180 kDa. Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser58 of human 14-3-3 ζ/δ protein.
Targets
14-3-3 gamma (Ser59) phosphate, 14-3-3 beta/alpha (Ser60) phosphate, 14-3-3 eta (Ser59) phosphate, 14-3-3 zeta/delta (Ser58) phosphate
Target Alias Names
YWHAG, YWHAB, YWHAH, YWHAZ, 14-3-3 gamma, 14-3-3 beta, 14-3-3 eta, 14-3-3 zeta
Isotype/Mimetic
IgG
Animal-Derived Biomaterials Used
BSA
Sequence Available
Yes
Original Discovery Method
Immunization
Antibody/Binder Origins
Animal-dependent discovery (in vitro display, OR immunisation pre-2020), In vitro recombinant expression, Animal-derived biomaterials used in production or final formulation