Description
Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb detects endogenous levels of histone H3 when di-methylated on Lys27. The antibody does show some cross-reactivity with mono-methylated Lys27, but does not cross-react with non-methylated or tri-methylated Lys27. Also, the antibody shows some cross-reactivity with histone H2B when di-methylated on Lys5. In addition, the antibody does not cross-react with mono-methylated, di-methylated or tri-methylated histone H3 Lys4, Lys9, Lys36 or histone H4 Lys20. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys27 is di-methylated.
Target
H3 (Lys27) dimethylate
Target Alias Names
H3C1, H3
Isotype/Mimetic
IgG
Animal-Derived Biomaterials Used
BSA
Sequence Available
Yes
Original Discovery Method
Immunization
Antibody/Binder Origins
Animal-dependent discovery (in vitro display, OR immunisation pre-2020), In vitro recombinant expression, Animal-derived biomaterials used in production or final formulation